Redirecting Lentiviral Vectors Pseudotyped with Sindbis Virus-Derived Envelope Proteins to DC-SIGN by Modification of N-Linked Glycans of Envelope Proteins ▿
Identifieur interne : 001459 ( Main/Exploration ); précédent : 001458; suivant : 001460Redirecting Lentiviral Vectors Pseudotyped with Sindbis Virus-Derived Envelope Proteins to DC-SIGN by Modification of N-Linked Glycans of Envelope Proteins ▿
Auteurs : Kouki Morizono ; Amy Ku ; Yiming Xie ; Airi Harui ; Sam K. P. Kung ; Michael D. Roth ; Benhur Lee ; Irvin S. Y. ChenSource :
- Journal of Virology [ 0022-538X ] ; 2010.
Abstract
Redirecting the tropism of viral vectors enables specific transduction of selected cells by direct administration of vectors. We previously developed targeting lentiviral vectors by pseudotyping with modified Sindbis virus envelope proteins. These modified Sindbis virus envelope proteins have mutations in their original receptor-binding regions to eliminate their natural tropisms, and they are conjugated with targeting proteins, including antibodies and peptides, to confer their tropisms on target cells. We investigated whether our targeting vectors interact with DC-SIGN, which traps many types of viruses and gene therapy vectors by binding to the N-glycans of their envelope proteins. We found that these vectors do not interact with DC-SIGN. When these vectors were produced in the presence of deoxymannojirimycin, which alters the structures of N-glycans from complex to high mannose, these vectors used DC-SIGN as their receptor. Genetic analysis demonstrated that the N-glycans at E2 amino acid (aa) 196 and E1 aa 139 mediate binding to DC-SIGN, which supports the results of a previous report of cryoelectron microscopy analysis. In addition, we investigated whether modification of the N-glycan structures could activate serum complement activity, possibly by the lectin pathway of complement activation. DC-SIGN-targeted transduction occurs in the presence of human serum complement, demonstrating that high-mannose structure N-glycans of the envelope proteins do not activate human serum complement. These results indicate that the strategy of redirecting viral vectors according to alterations of their N-glycan structures would enable the vectors to target specific cells types expressing particular types of lectins.
Url:
DOI: 10.1128/JVI.00435-10
PubMed: 20484510
PubMed Central: 2898243
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000721
- to stream Pmc, to step Curation: 000721
- to stream Pmc, to step Checkpoint: 000840
- to stream Ncbi, to step Merge: 000212
- to stream Ncbi, to step Curation: 000212
- to stream Ncbi, to step Checkpoint: 000212
- to stream Main, to step Merge: 001461
- to stream Main, to step Curation: 001459
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Redirecting Lentiviral Vectors Pseudotyped with Sindbis Virus-Derived Envelope Proteins to DC-SIGN by Modification of N-Linked Glycans of Envelope Proteins <xref ref-type="fn" rid="fn2">▿</xref>
</title>
<author><name sortKey="Morizono, Kouki" sort="Morizono, Kouki" uniqKey="Morizono K" first="Kouki" last="Morizono">Kouki Morizono</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ku, Amy" sort="Ku, Amy" uniqKey="Ku A" first="Amy" last="Ku">Amy Ku</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Xie, Yiming" sort="Xie, Yiming" uniqKey="Xie Y" first="Yiming" last="Xie">Yiming Xie</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Harui, Airi" sort="Harui, Airi" uniqKey="Harui A" first="Airi" last="Harui">Airi Harui</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kung, Sam K P" sort="Kung, Sam K P" uniqKey="Kung S" first="Sam K. P." last="Kung">Sam K. P. Kung</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Roth, Michael D" sort="Roth, Michael D" uniqKey="Roth M" first="Michael D." last="Roth">Michael D. Roth</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lee, Benhur" sort="Lee, Benhur" uniqKey="Lee B" first="Benhur" last="Lee">Benhur Lee</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chen, Irvin S Y" sort="Chen, Irvin S Y" uniqKey="Chen I" first="Irvin S. Y." last="Chen">Irvin S. Y. Chen</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">20484510</idno>
<idno type="pmc">2898243</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2898243</idno>
<idno type="RBID">PMC:2898243</idno>
<idno type="doi">10.1128/JVI.00435-10</idno>
<date when="2010">2010</date>
<idno type="wicri:Area/Pmc/Corpus">000721</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000721</idno>
<idno type="wicri:Area/Pmc/Curation">000721</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000721</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000840</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000840</idno>
<idno type="wicri:Area/Ncbi/Merge">000212</idno>
<idno type="wicri:Area/Ncbi/Curation">000212</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000212</idno>
<idno type="wicri:doubleKey">0022-538X:2010:Morizono K:redirecting:lentiviral:vectors</idno>
<idno type="wicri:Area/Main/Merge">001461</idno>
<idno type="wicri:Area/Main/Curation">001459</idno>
<idno type="wicri:Area/Main/Exploration">001459</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Redirecting Lentiviral Vectors Pseudotyped with Sindbis Virus-Derived Envelope Proteins to DC-SIGN by Modification of N-Linked Glycans of Envelope Proteins <xref ref-type="fn" rid="fn2">▿</xref>
</title>
<author><name sortKey="Morizono, Kouki" sort="Morizono, Kouki" uniqKey="Morizono K" first="Kouki" last="Morizono">Kouki Morizono</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ku, Amy" sort="Ku, Amy" uniqKey="Ku A" first="Amy" last="Ku">Amy Ku</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Xie, Yiming" sort="Xie, Yiming" uniqKey="Xie Y" first="Yiming" last="Xie">Yiming Xie</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Harui, Airi" sort="Harui, Airi" uniqKey="Harui A" first="Airi" last="Harui">Airi Harui</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kung, Sam K P" sort="Kung, Sam K P" uniqKey="Kung S" first="Sam K. P." last="Kung">Sam K. P. Kung</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Roth, Michael D" sort="Roth, Michael D" uniqKey="Roth M" first="Michael D." last="Roth">Michael D. Roth</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lee, Benhur" sort="Lee, Benhur" uniqKey="Lee B" first="Benhur" last="Lee">Benhur Lee</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chen, Irvin S Y" sort="Chen, Irvin S Y" uniqKey="Chen I" first="Irvin S. Y." last="Chen">Irvin S. Y. Chen</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Redirecting the tropism of viral vectors enables specific transduction of selected cells by direct administration of vectors. We previously developed targeting lentiviral vectors by pseudotyping with modified Sindbis virus envelope proteins. These modified Sindbis virus envelope proteins have mutations in their original receptor-binding regions to eliminate their natural tropisms, and they are conjugated with targeting proteins, including antibodies and peptides, to confer their tropisms on target cells. We investigated whether our targeting vectors interact with DC-SIGN, which traps many types of viruses and gene therapy vectors by binding to the N-glycans of their envelope proteins. We found that these vectors do not interact with DC-SIGN. When these vectors were produced in the presence of deoxymannojirimycin, which alters the structures of N-glycans from complex to high mannose, these vectors used DC-SIGN as their receptor. Genetic analysis demonstrated that the N-glycans at E2 amino acid (aa) 196 and E1 aa 139 mediate binding to DC-SIGN, which supports the results of a previous report of cryoelectron microscopy analysis. In addition, we investigated whether modification of the N-glycan structures could activate serum complement activity, possibly by the lectin pathway of complement activation. DC-SIGN-targeted transduction occurs in the presence of human serum complement, demonstrating that high-mannose structure N-glycans of the envelope proteins do not activate human serum complement. These results indicate that the strategy of redirecting viral vectors according to alterations of their N-glycan structures would enable the vectors to target specific cells types expressing particular types of lectins.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Chen, Irvin S Y" sort="Chen, Irvin S Y" uniqKey="Chen I" first="Irvin S. Y." last="Chen">Irvin S. Y. Chen</name>
<name sortKey="Harui, Airi" sort="Harui, Airi" uniqKey="Harui A" first="Airi" last="Harui">Airi Harui</name>
<name sortKey="Ku, Amy" sort="Ku, Amy" uniqKey="Ku A" first="Amy" last="Ku">Amy Ku</name>
<name sortKey="Kung, Sam K P" sort="Kung, Sam K P" uniqKey="Kung S" first="Sam K. P." last="Kung">Sam K. P. Kung</name>
<name sortKey="Lee, Benhur" sort="Lee, Benhur" uniqKey="Lee B" first="Benhur" last="Lee">Benhur Lee</name>
<name sortKey="Morizono, Kouki" sort="Morizono, Kouki" uniqKey="Morizono K" first="Kouki" last="Morizono">Kouki Morizono</name>
<name sortKey="Roth, Michael D" sort="Roth, Michael D" uniqKey="Roth M" first="Michael D." last="Roth">Michael D. Roth</name>
<name sortKey="Xie, Yiming" sort="Xie, Yiming" uniqKey="Xie Y" first="Yiming" last="Xie">Yiming Xie</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001459 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001459 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV2 |flux= Main |étape= Exploration |type= RBID |clé= PMC:2898243 |texte= Redirecting Lentiviral Vectors Pseudotyped with Sindbis Virus-Derived Envelope Proteins to DC-SIGN by Modification of N-Linked Glycans of Envelope Proteins ▿ }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20484510" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a CovidV2
This area was generated with Dilib version V0.6.33. |